Generating Power Like Plants

When plants absorb sunlight, they convert carbon dioxide into energy-rich organic compounds. What if humans could do the same thing? What if we could pull CO2 out of the air and use it to build organic molecules? This revolutionary idea is still just that — an idea. But organic chemists at UNC are laying the groundwork for turning it into reality.

a blacklight makes everything purple while a gloved hand places a test tube full of yellow fluid into a a test tube trayphoto by Mary Lide Parker
A graduate student sets up a light-driven reaction with an organic dye.
January 11th, 2018

A freshwater aquarium looks a bit like an underwater garden — bright, green grasses grow next to burgundy leaves resembling arugula. Small, silver fish dart between the foliage, as streams of bubbles flow up through the water column.

Dave Nicewicz stares at this array of life contained within the rectangular glass tank on his desk.

“Look at the little oxygen bubble at the edge of the leaf — that’s a good one” he says, pointing to the plant with a reddish hue. A small heat lamp mimics the sun, radiating light into the tank, while six different species of plants release oxygen.

“I love this thing because you can actually see photosynthesis happening — you can’t see that outside.”

Dave Nicewicz points to the oxygen bubbles in his freshwater aquarium.

Dave Nicewicz points to the oxygen bubbles in his freshwater aquarium.

As a research scientist, Nicewicz finds inspiration in photosynthesis. And while he loves plants (both his office and home are full of them) he is not a biologist — he’s an organic chemist.

“I’m not equating what we do to photosynthesis, but we’re inspired by that process,” he says.

Nicewicz’s ultimate goal is to use the most abundant source of energy on the planet — sunlight — to power chemical reactions. “To harvest sunlight in a way that either directly or indirectly can be used in chemical reactions by translating photons into electrons,” he says. “That’s the future.”

Humans have not yet engineered a way to create the complex biological machinery of plant life in a lab, according to Nicewicz, but the inspiration for the starting point is there — in the form of photo-redox catalysis.

“It’s just a fancy way of saying we use light to make cool molecules,” says Nate Romero, a former PhD student of the Nicewicz lab.

To explain this chemical process, it’s helpful to break down each word. We’re talking about creating reactions, and all reactions require an energy input – like heat or light. Photo refers to light, and redox refers to the transfer of electrons. Catalysis simply means using a catalyst, which decreases the amount of energy needed for a reaction to occur.

“We can use a single catalyst to make hundreds or thousands or millions of molecules that we’re interested in studying,” says Cole Cruz, a graduate research assistant in the lab.

By trying out different catalysts, this group of organic chemists can trim off one part of a molecule, or combine molecules that wouldn’t typically combine.  These “cool molecules” play a major role in the development of vital plastics and medicines around the world — making the production process more economical and more environmentally friendly.

To do that, Nicewicz and his students want to avoid the use of toxic metals, and utilize processes that generate little or not waste at all — the same way plants generate energy. “That’s the challenge for chemical synthesis in the 21st century,” he says. “We have to mimic how plants make molecules.”

Light it up  

While scientists have been studying photo-chemistry over a century, photo-redox catalysis hasn’t received mainstream attention until the past decade or so. Advances in technology, especially the widespread use of LEDs, and the ability to pinpoint precise wavelengths of light, have made it more commonplace, according to Nicewicz.

More accessible and less expensive than lasers, LEDs are highly useful for organic chemistry because a researcher can utilize a particular color of light in a reaction.

Researchers in the Nicewicz lab need highly energetic yet tiny wavelengths of light to power their reactions. They measure those wavelengths in nanometers — or one-billionth of a meter. A sheet of paper is about 100,000 nanometers thick. A strand of human DNA is 2.5 nanometers in diameter.

“The output of these things is really narrow,” Nicewicz says. “Typically, a wavelength of 20 nanometers gives you pretty precise control.”

That precision is key for pinpointing the strongest reactions. Suppose a researcher has four components in a solution — if she uses thermal energy, then all four parts will be heated, which can lead to unwanted side reactions and wasted energy. By using light, the researcher has much more control.

“Perhaps one of them absorbs light at a specific wavelength that is different from all the others,” Nicewicz says. “You can input energy into that one particular component in the presence of all the others, which means a selective reaction can take place — that’s what we’re getting at.”

They’re not using plants in these reactions though. The lab uses a synthetic version of the pigments founds in plants — in the form of an organic dye. “There are some parallels to the dyes found naturally in plants, like chlorophyll, but ours are much more simplified,” Nicewicz says.

When an organic molecule inside that dye absorbs light, the molecule moves into a higher energy state, transporting electrons around, and creating a more powerful reaction to fuel production. The key for researchers in the Nicewicz lab is finding the perfect combination of color and light.

Concoct a color

Organic dyes have been around for thousands of years. “Purple dye is probably one of the most prized possessions of the ancient world — that was actually an accidental discovery,” Nicewicz says.

But the organic dyes that his lab produces are no accident. While dyes can come in many different colors, the Nicewicz lab mainly targets yellow and orange because they can absorb the highest energy light, and then put that energy to work in reactions. The dyes are easy to make using readily abundant, organic raw materials. Researchers combine extracts from essential oils — almond extract for example — with sulfuric acid in a dehydration reaction to create the powdery dye.

Dyes that students have created in the lab.

Dyes that students have created in the lab.

Nicewicz is designing a new undergraduate lab section for second-year organic chemistry students to produce libraries of these dyes. He piloted the program during the 2017 summer session, then introduced a larger-scale version during the fall semester.

“We have 200 undergraduates making these dyes,” Nicewicz says. “It’s easy chemistry, and they’re using household items.”

The process involves designing simple reactions that students can complete in a single step. They pick different points to vary within the structure of the dye, and in the process, invent many slightly different dyes. “Then we can evaluate those to see which combinations produce better reactivity,” Nicewicz says. “In the first round of these we’re hoping to generate about 500 structures per laboratory.”

Turn it on   

The goal is to produce thousands of structures, and eventually find one that is really good. “This in an iterative process,” Nicewicz says. “We find the winners from one set, take what we think are the most valuable properties of those, and then explore those properties farther in the next set so that we’re constantly refining — it’s like an algorithm.”

Another one of Nicewicz’s long-term goals is to produce all these catalysts in a reusable fashion, utilizing a flow set up with the catalyst coating the walls of a reactor. “We flow our reactants through that, the reaction happens, and then we can recycle this catalyst indefinitely,” he says.

“We’re constantly looking for new reactions, and for ways to improve on old ones,” Romero says. “Ultimately, we hope these tools we’ve developed from photo-redox catalysis can be useful for other chemists.”

And indeed, they have.

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While much of their work focuses on creating new molecules, the Nicewicz lab also produces aromatic compounds. (For those outside the chemistry world, aromatic refers not just to smell but to a set of compounds with a distinctive stable structure.) The best known aromatic compound, which has a sweet, gasoline-like odor, is benzene. “Most pharmaceuticals and agro-chemicals have a benzene embedded in them somewhere,” Nicewicz says.

Researchers start with the benzene core structure, and build off of that to make more complex structures. These compounds can be used to make anilines, which are used to create polyurethane coatings and medicines like Tylenol.

Pharmaceutical companies often call this research “discovery” or “medicinal” chemistry to find new compounds. “They want reactions they can do really easily to make thousands of molecules and then they screen those thousands of molecules against certain disease types,” Nicewicz says. A process similar to that of Nicewicz’s students begins — build up a library of compounds, find the winners, and then keep modifying them until you find the best possible one.

“They’re already using some of our science because it gets them to molecular structures in a much more concise manner than they have been previously,” Nicewicz says. “In some cases, they make molecules that they can’t make in any other way.”

Pharmaceutical companies like Merck, BMS, Novartis, and AbbVie use chemistry developed in the Nicewicz lab. But Nicewicz and his students continue to address problems like how to flow the chemicals past the light to get the desired reaction. “These problems will be solved in the long run,” Nicewicz says. “They just require a little bit of hard work.”

Build for the future

While photosynthesis began roughly 3 billion years ago, the planet was not covered in forests until 500 million years ago. Nicewicz hopes humans can refine a synthetic version of photosynthesis a bit faster than that.

“There is so much that goes into being able to synthesize molecules in ways that are as complex as what plants do,” Nicewicz says. The work of his lab and others in the field of organic chemistry is just one part of it — we also need advances in physics, engineering, and biology.

And it’s not just a matter of using sunlight. Nicewicz wants to utilize other naturally occurring energy feedstocks.

“We can burn petroleum, or we can use it to make valuable medicines and materials—I think that’s what we should be doing to take better advantage of this valuable resource,” Nicewicz says. “Instead we’re just burning it and causing more problems. What my lab is trying to do is take those abundant resources and turn those into chemicals that we need on an everyday basis.”

Back in his office, Nicewicz envisions a future where this process could exist, and the impact it would have.

“It would mean that we don’t have to extract oil from the ground. It would mean that we don’t even have to process biomass, which is energy-intensive,” he says. “If we could just pull CO2 out of the air and selectively insert those carbon atoms where we want in a molecule, that would mean we’re just as good as plants — but we’re not yet.”

Nicewicz turns back to the aquarium, and smiles at the percolating oxygen bubbles. “It’s pretty incredible that plants can do that.”

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Dave Nicewicz is an associate professor and director of graduate studies in the Chemistry department.

Cole Cruz is a graduate research assistant in the Nicewicz lab.

Nate Romero is a postdoctoral fellow in the Swager Group at MIT.