Crouching Researcher, Hidden Sparrow

Four students spent their summer in Colorado stalking Lincoln’s sparrows in search of answers to fundamental biological questions.

PhD student Emma Reinhardt and junior Torin O'Brien collect data on birds in a meadow at the base of the San Juan Mountains near Silverton, Colorado. (photo by Alyssa LaFaro)
September 27th, 2022

In the San Juan Mountains near Silverton, Colorado, four UNC-Chapel Hill students wake at 5 a.m. to melodic phone alarms and amble out of their dew-soaked tents. They sleepily shimmy into rainproof jackets, pants, and knee-high rubber boots. They heat water for tea and munch on Pop-tarts and energy bars while they wait. With steamy mugs in hand, they clamber into their white van to head to the field site a half-mile down the road.

Armed with notebooks, binoculars, and a directional microphone, they hop on the Colorado Trail near Molas Pass and make a 10-minute trudge through shin-high grasses slick with frost before plopping down in a meadow. Then, they listen for birdsong.

This is the first of many activities the students will engage in throughout the day. They’re here as part of biologist Keith Sockman’s lab, collecting data on elusive Lincoln’s sparrows.

For Sockman and his lab, Lincoln’s sparrows are a goldmine of information. But getting to them isn’t easy. Their nesting site sits at nearly 11,000 feet, so the air is thin and, like most mountain ecosystems, the weather is unpredictable — even in summer. The small brown birds with striped heads live on the ground at the base of chest-high woody bushes in an open wetland. Hunting for their tiny nests can be a grueling, hours-long process.

Sockman has been studying this population of sparrows since 2005 and has made multiple discoveries during that time. Egg order, for example, matters. Lincoln’s sparrows typically lay one egg per day for four days. The first egg is least likely to hatch, while the fourth is least likely to fledge the nest. Hatching order also predicts bill shape. Last-hatched nestlings have wider, flatter bills than their older siblings, which can make their songs less attractive to potential mates.

Lincoln's sparrow leaps off a branch

A Lincoln’s sparrow in mid-flight. (photo by Alyssa LaFaro)

But the most important discovery, according to Sockman, is that a female Lincoln’s sparrow will modify the size of her egg based on how previous eggs performed — which shows their ability to adapt, something called plasticity.

“By studying one population for a long time, I am able to make more ecologically important discoveries,” Sockman says.

This year, two of Sockman’s PhD students are collaborating with him and running their own projects in the process. Emma Reinhardt is recording male birdsong and remixing it randomly to study its evolution and determine if the order of notes in a song matters. Nico Frasson is collecting tissue samples to identify where the birds migrate in the winter to learn how migration affects health, survival, and breeding success. They are accompanied by two undergraduates— junior Torin O’Brien and senior Colleen Whitener.

The team spent six weeks primitive camping through hard rains and cold nights, waking up in the pre-dawn hours to record as much as they could about these little brown birds.

The bird DJ

“Over there!” Reinhardt whispers excitedly to the group.

In unison, they lift their binoculars. A male Lincoln’s sparrow has just flown into a nearby tree. Reinhardt creeps across the meadow to a neighboring bush and hides a turquoise speaker among the branches. After rejoining her teammates, she signals to Whitener to start recording and presses “play” on her iPhone. Birdsong emanates from the small speaker and eventually, after a minute or two, the male sparrow flies down to the bush and listens. Then, he begins to sing a beautiful, complex song.

This is Reinhardt’s second field season at Molas Pass, and she couldn’t be more excited. She began her PhD program at UNC-Chapel Hill in 2020 — a time when she didn’t know what the next day would bring, let alone when a field season would happen. During that period, Sockman suggested she begin listening to his past recordings of Lincoln’s sparrows and how they use syllables, the repeating segments within their birdsong.

Emma Reinhardt records birdsong in the field

Emma Reinhardt records birdsong in the field using a directional microphone. (photo by Alyssa LaFaro)

One song contains anywhere from four to eight different syllable types, which can vary in the number of times they’re repeated and their position in the song. As Reinhardt dove into decades-worth of Sockman’s data, she grew more and more entranced. At first, she wanted to know how the syllables changed over time and began creating a lexicon of the birdsong.

“It’s almost like an evolutionary tree for the sounds these birds are making,” she says.

This stimulated more and more questions. Are new syllables appearing in later years? And if so, are they similar to the ones from previous years?

“I noticed that some of these syllable types will only occur in the first part of a song, or they’ll only occur adjacent to another syllable type. I found that interesting — that it was so highly stereotyped. Even though there’s a high variability between songs and between males, they tend to stick to the same syntax.”

Syntax is how words and phrases are arranged to form a sentence. Reinhardt wants to know if syntax matters in birdsong and how females’ mate selection influences both sexual and cultural evolution.

“There is some evidence of syntax in songbirds, but it’s understudied and not well understood,” she explains. “I think there’s a general assumption that birdsong lacks complexity, that birds are just kind of repeating things they know, and that order doesn’t necessarily have meaning. It would be interesting if we could show that order really does matter, and that syntax conveys salient information for when females are selecting mates.”

To study this, Reinhardt remixes previously recorded birdsong from Lincoln’s sparrows using a random number generator. She also uses a standard, unmixed recording that acts as a control. Then, out in the field, she plays these tracks — the scrambled song on one day and the control on another — and records the responses and behaviors of male Lincoln’s sparrows.

Reinhardt delights in the fact that the sparrows are responding to the recordings at all. She wasn’t sure her experiment would work. Now back in Chapel Hill, she is analyzing the recordings she collected. In the spring, she hopes to trap a few Lincoln’s sparrows as they migrate through North Carolina so that she can begin observing them in the lab.

“I have read a couple papers where people make a strong argument that the way birds learn songs is similar to the way humans learn language,” Reinhardt shares.  “It can be a comparison, almost like a framework, for development. While we can’t experiment on humans, we can study birdsongs to learn more about how the brain works when it’s learning something language-like.”

Emma Reinhardt, Nico Frasson, and Torin O'Brien look for birds
Lincoln's sparrow
a pair of binoculars
Nico Frasson and Emma Reinhardt look for birds
Lincoln's sparrows eggs
baby Lincoln's sparrows
Reinhardt takes takes measurements of a bird's body size.
Emma Reinhardt and Torin O'Brien at the field site
A view of a mountain top near Spencer Peak reflects in a parking lot puddle
Torin O’Brien, Nico Frasson, Colleen Whitener, and Emma Reinhardt.
the student's campsite
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The migration detective

Each morning, Frasson is the first one out of his tent — up about 15 minutes earlier than his fellow researchers — to soak in the quiet. He uses this extra time to gather his thoughts and make tea for the team, which he hand-delivers to each member before they depart the campsite.

That same thoughtfulness follows him into the field. Oftentimes, while the others are huddled together to make observations, Frasson strays a few feet away to cover more ground, in search of sparrows outside of the group’s immediate vicinity.

After completing his biology degree in France, Frasson took a gap year — “well, really five years,” he clarifies with a laugh — to explore his interests and figure out if he wanted to attend graduate school. During that time, he worked with labs across France, Italy, Canada, and the United States, studying a variety of animals, from small fish called sticklebacks to elephant seals.

Nico Frasson searches for birds

Nico Frasson searches for nesting sites in the field. (photo by Alyssa LaFaro)

Now a first-year PhD student at Carolina, Frasson focuses on physiology, behavior, and speciation — how populations diverge to create new species. He studies this in Lincoln’s sparrows via their migration patterns, which can provide information about health and breeding. To uncover where these birds migrate to in winter, Frasson collects toenail clippings, feathers, and blood for isotope analyses.

Isotopes are made up of two or more forms of the same element and are in everything — from the food we eat to the air we breathe. This means they’re a reflection of the environment an organism spends time in and can be traced through the tissue samples Frasson collects.

Frasson and Reinhardt capture Lincoln’s sparrows in two ways. The first involves placing 50 traps along a 1.5-mile route that encircles the field site and resides near nesting areas. Each morning around 7:30 a.m., when Reinhardt wraps up her birdsong experiments, the undergraduates head out to open the traps and bait them with birdseed.

On a good day, they catch four to five Lincoln’s sparrows. When they do, they carefully remove them from the trap and check whether the bird has been banded — a bracelet with a federal identification number. If it hasn’t, they give it one so they can track it over the course of its life and upload the data they collect to the U.S. Bird Banding Laboratory’s website.

They proceed to take a series of measurements including weight and the size of their beak, legs, wings, and tail feathers. Once they’ve recorded these stats, Frasson clips a few toenails, collects two tail feathers, and carefully draws a small vial of blood for analysis. Then, he splays his palms, and the bird soars into the sky.

For birds that elude these traps, the researchers use a mist net — a lightweight net they can place anywhere within the field site. This helps them target specific birds, like the parents of a nest they’ve been monitoring. At first, they wait patiently to see if the birds will fly into the net on their own, and if that doesn’t work, they try to chase them into it.

After a successful field day — which lasts anywhere from six to 10 hours — Frasson returns to the campsite to process his tissue samples. He pulls large, shallow boxes overflowing with glass vials out of a shared trailer and adds his specimens from the day. He pops the hood on the passenger van and hooks up a battery big enough to power a centrifuge, which spins the blood to separate red blood cells used to identify the stable isotope signature of the birds. After 15 minutes, he removes the samples and stores them in the fridge.

Then, it’s time to relax. Taking a break from research is key to a successful work-life balance while in the field, according to Frasson.

Everyone heads to Reinhardt’s trailer to play cards, but they don’t totally escape thoughts of their flying friends — the deck features a dozen North American hummingbirds.

Keith Sockman is an associate professor in the Department of Biology within the UNC College of Arts and Sciences.

Emma Reinhardt is a second-year PhD student and a member of the Sockman Lab in the Department of Biology within the UNC College of Arts and Sciences. For this project, she received a Summer Research Fellowship through The Graduate School.

Nico Frasson is a first-year PhD student and a member of the Sockman Lab in the Department of Biology within the UNC College of Arts and Sciences.